Erik Stifler (John White) has a difficult time living up to his family name. He is Steve and Matt Stifler's cousin. He is perhaps the only Stifler about to graduate from high school as a virgin. The film opens with Erik feigning illness so that he can stay home and masturbate. Unfortunately, just as he is about to ejaculate, his parents and grandmother unexpectedly walk into the door and are hit with Erik's semen, after which his grandmother dies of a heart attack. Erik's dad later surmises that, as a Stifler, his son should be out having sex instead of masturbating. Erik's girlfriend of two years, Tracy (Jessy Schram), loves him, but is not ready for intercourse.Later that same evening Tracy decides to have sex and she calls Erik to come on over to her house to do the deed. But their first attempt goes horribly wrong when her father shows up in the living room and Erik is forced to run, stark naked, home when he gets discovered. The next morning, when Erik has talk with Tracy about another attempt, she backs out of trying again.Erik's friends Cooze (Jake Siegel) and Ryan (Ross Thomas) plan a road trip to visit Erik's cousin Dwight Stifler (Steve Talley) at Michigan State University during an event known as the Naked Mile. Tracy sees this as an opportunity to give Erik a "guilt free weekend pass," hoping that he can quench his lust and get sex out of his system since she is not ready. But after attending her weekly yoga class, Tracy has a run-in with Jim's Dad, Noah Levenstein (Eugene Levy) whom she confides in about her situation with Erik. Mr. Levenstein persuades her not to give up on what she and Erik have.As soon as Erik and his friends arrive on campus, they witness an over-the-top drinking contest where Dwight is crowned a campus champion. Later, they lose a rough game of football against a bitter rival fraternity composed almost entirely of midgets, led by their ill-tempered leader known as Rock (Jordan Prentice). Dwight, Erik and the guys end up in a brawl with the same midgets on several occasions.The first night when the guys are at a college bar, Erik meets a college girl named Brandy (Candace Kroslak) who has a fetish for virgin boys, prompting Ryan and Cooze to make a bet with each other that Erik won't sleep with Brandi and will remain a virgin. At the same time, Ryan and Cooze end up getting turned down by a couple of girls they have met at the bar, Jill (Jaclyn A. Smith) and Alexis (Angel Lewis), who are taken by a couple of the midgets.The following morning, Dwight gets jumped by Rock and his midget fraternity in disguise while walking down the street, landing him in the hospital. Dwight tells the guys he probably won't make it to the Naked Mile, but yet still manages to make it right in time. Right away, he joins up with Erik, Ryan, and Cooze, who are at first reluctant to run, but when Brandy, Jill, and Alexis show up to run with the guys, they're finally prompted to strip down and run with the girls, and end up having a great time. Mr. Levenstein oversees the event and cheers the students on.Finally as Erik and Brandy reach the finish line, they steal a kiss, which is then caught on camera for a news report on TV. Watching the news report about the Naked Mile back at home, Tracy is upset and feels guilty that she allowed Erik the free pass. Her friends Brooke and Natalie convince her to also lose her virginity before he gets back.Later that evening, Erik realizes that he loves Tracy, confesses to Brandy that he can't sleep with her, and rushes back to see his girlfriend at East Great Falls. However, Erik's SUV runs out of gas and he is forced to steal a horse and ride barebacked into town. When he gets to her house, Tracy's dad says she is at a party and Erik arrives at the party just as Tracy has headed upstairs, presumably to lose her virginity to her ex-boyfriend. Erik loudly pounds on the closed bedroom door, proclaiming his love for her. However, Tracy was not in the room because she had decided that she could not go through with her plans. The two decide that they should be each other's first, and they make love.During the post-Naked Mile party, Dwight spots Vicky (Mika Winkler), the midget girlfriend of Rock, and the two of them head up to Dwight's room to have sex. Also at the Beta house, Cooze makes with with Jill who ties him up and subjects him to S&M and bondage. Ryan has more pleasant luck when he has sex with Alexis.When Erik returns to the Beta house to pick up his friends the next morning, each boy shares stories of his experiences from the night before. The guys then ask Erik if he "sealed the deal" with Brandi that night, and Erik tells them no, prompting Ryan and Cooze to pay up on their bet, until Erik tells them about his adventure back home to make up to Tracy and finally lose his virginity. The guys are now proud of Erik for officially living up to the Stifler family name, and the three friends finally drive back to East Great Falls.In the final scene, Dwight send a DVD to Rock that reads, "Payback's a bitch!" It reveals both Dwight and Vicky having sex, as Rock yells out, "Stifler!" As it turns out, Dwight and Vicky start dating, and they end up traveling the world together, as various postcard pictures show their many journeys together as the end credits roll.